| 產(chǎn)品名稱 |
delta TA4-1 [dTA4-1] |
| 商品貨號(hào) |
B164345 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human delta heavy chain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human delta heavy chain |
| Comments |
Animals were immunized with human delta heavy chain. Spleen cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG3; kappa light chain |
| Name of Depositor |
JF Kearney, H Kubagawa |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
. . J. Clin. Immunol. 2: 942-945, 1982.
Kubagawa H, et al. Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence. J. Clin. Immunol. 2: 264-269, 1982. PubMed: 6815220
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