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DS-1
DS-1
規(guī)格:
貨期:
編號(hào):B164370
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) DS-1
商品貨號(hào) B164370
Organism Homo sapiens, human
Tissue derived from metastatic site: pleural effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphangiectasia
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
Derivation
The DS-1 cell line was established from cells in the pleural effusion from an immunodeficient patient with intestinal lymphangiectasia.


Antigen Expression
HLA Class I, (100%); HLA Class II (38%); plasma cell antigen (PCA) (99%); CD2 (<5%); CD3 (<5%); CD4 -; CD5 -; CD7 -; CD8 -; CD20 -; CD30 -; CD38 -; CD45 -
Receptor Expression
interleukin 6 (IL-6)
Genes Expressed
surface immunoglobulin
Cellular Products
surface immunoglobulin
Comments
DS-1 is IL-6 dependent.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 10 U/ml IL-6, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 cells/mL. Maintain cultures between 1 x 105 and 2 x 106 cells/mL. Recovery of this line from the frozen state is improved by using a feeder layer of irradiated MRC-5 cells (ATCC 55-X).
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9,10
D5S818: 11,14
D7S820: 8,14
THO1: 6,9.3
TPOX: 9,11
vWA: 18,19
Isotype IgG; kappa light chain
Name of Depositor The United States of America
U.S. Patent Number
References

Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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