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DU4475
DU4475
規(guī)格:
貨期:
編號(hào):B164380
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DU4475
商品貨號(hào) B164380
Organism Homo sapiens, human
Tissue mammary gland; breast; derived from metastatic site: skin carcinoma
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease carcinoma, skin
Age 70 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 93; range = 85 to 95.
The cell line is aneuploid human female, with chromosome counts in the tetraploid range. Normal chromosomes N16 and N21 are least well represented, with from none to two copies per karyotype, while chromosome N7 tends to be over-represented, with four or five copies per karyotype, whereas two to four copies are found of most of the remaining normal chromosomes. Twenty-two marker chromosomes are found including: iso(1q), del(1)(q32), der(1)t(1;?)(p36;?), del(3)(q24), der(3)t(3;?)(q24;?), del(3)(p14p25), t(6q;21?) and others. Eight are identical to those reported or portrayed in the report by A.J. Langlois, et al.
Images
Clinical Data
70 years
Caucasian
female
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
Numerous desmosomes and interdigitating microvilli were observed by transmission electron microscopy.
This line grows in floating clusters and viability cannot be determined accurately with dye exclusion tests.
The cells form transplantable tumors similar in histology to that of the original carcinoma.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 2 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,12
D13S317: 11,14
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 6,8
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1-2
PGM3, 1
Name of Depositor AJ Langlois
Deposited As Homo sapiens
References

Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

Langlois AJ, et al. Morphological and biochemical properties of a new human breast cancer cell line. Cancer Res. 39: 2604-2613, 1979. PubMed: 221108

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