| 產(chǎn)品名稱 |
5A HS MYC |
| 商品貨號 |
B164383 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Tissue |
ovary |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent, adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Derived from the parental cell line - CHO-DUKKX BII |
| Clinical Data |
female |
| Genes Expressed |
over produces mouse c-myc protein when heated |
| Cellular Products |
over produces mouse c-myc protein when heated |
| Comments |
The cells are reported to contain DNA sequences from mouse, drosophila, adenovirus and E. coli. |
| Complete Growth Medium |
Alpha modified Eagle's minimum essential medium with 0.32 mM methotrexate, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: culture medium ,95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
R Kingston |
| Deposited As |
Cricetulus griseus |
| U.S. Patent Number |
European Patent No.: EP0232845 A2 |
| References |
Wurm FM, et al. Inducible overproduction of the mouse c-myc protein in mammalian cells. Proc Natl Acad Sci 83(15): 5414-5418, 1986. PubMed: 3526329
|
