国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > E.G7-OVA [derivative of EL4]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
E.G7-OVA [derivative of EL4]
E.G7-OVA [derivative of EL4]
規(guī)格:
貨期:
編號(hào):B164385
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 E.G7-OVA [derivative of EL4]
商品貨號(hào) B164385
Organism Mus musculus, mouse
Tissue lymphocyte
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain C57BL/6N
Applications
This cell line is a model system for studying major histocompatibility complex (MHC) class I restricted responses of cytotoxic T lymphocytes in mice.
Storage Conditions liquid nitrogen vapor phase
Derivation
E.G7-OVA was derived in 1988 from the C57BL/6 (H-2 b) mouse lymphoma cell line EL4 (ATCC TIB-39).
The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene.
Antigen Expression
H-2 b
Genes Expressed
chicken ovalbumin
Comments
E.G7-OVA cells contain a single copy of the inserted plasmid and synthesize and secrete OVA constitutively.
C57BL/6 mice immunized with E.G7-OVA cells give rise to H-2 Kb restricted cytotoxic lymphocytes specific for the OVA 258-276 peptide.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor MJ Bevan
Deposited As Mus musculus
Year of Origin 1988
References

Moore MW, et al. Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777-785, 1988. PubMed: 3261634

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479