產(chǎn)品名稱 |
EB-3 [EB3] |
商品貨號 |
B164392 |
Organism |
Homo sapiens, human |
Cell Type |
B lymphocyte |
Product Format |
frozen |
Morphology |
lymphoblast |
Culture Properties |
suspension |
Biosafety Level |
2 [Cells Contain HERPESVIRUS]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
Burkitt's lymphoma |
Age |
3 years |
Gender |
Male |
Ethnicity |
Black |
Storage Conditions |
liquid nitrogen vapor phase |
Images |
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Clinical Data |
male Black 3 years |
Antigen Expression |
HLA A3, Aw32, Cw2 |
Virus Resistance |
partially resistant to polioviruses and vesicular stomatitis virus |
Comments |
EBNA positive. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
Subculturing |
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3 x 105 viable cells/mL. |
Cryopreservation |
culture medium, 90%; DMSO, 10% |
Culture Conditions |
Temperature: 37.0°C
Atmosphere: air, 95%. carbon dioxide (CO2), 5% |
STR Profile |
Amelogenin: X,Y CSF1PO: 12,15 D13S317: 12,14 D16S539: 10,12 D5S818: 9,10 D7S820: 11 THO1: 7 TPOX: 6,9 vWA: 17,19 |
Isoenzymes |
G6PD, A |
Name of Depositor |
W Henle |
Deposited As |
Homo sapiens |
References |
Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
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