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EJ-6-2-Bam-6a
EJ-6-2-Bam-6a
規(guī)格:
貨期:
編號:B164400
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EJ-6-2-Bam-6a
商品貨號 B164400
Organism Mus musculus, mouse
Tissue embryo
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Carcinoma
Age embryo
Strain NIH/Swiss
Applications
This line was established by C. Shih et al.
Derivation
This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ).
Tumorigenic Yes
Effects
Yes, forms subcutaneous tumors in BALB/c and NSF mice
Comments
This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ).
The cells are morphologically transformed and highly tumorigenic in BALB/c and NSF mice.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.
Name of Depositor RA Weinberg
Deposited As Mus musculus
Year of Origin 1981
References

Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109

Shih C, et al. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature 290: 261-264, 1981. PubMed: 7207618

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