| 產(chǎn)品名稱(chēng) |
F1-3G8-1 |
| 商品貨號(hào) |
B164422 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the F1 antigen of plague bacillus and may be of use as a diagnostic reagent. The antibody has been used to establish an ELISA method for use in quantitating the F1 antigen. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bubonic plague bacillus; Yersinia pestis (F1 antigen) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bubonic plague bacillus; Yersinia pestis (F1 antigen) |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with plague bacillus. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody is specific for the F1 antigen of plague bacillus and may be of use as a diagnostic reagent. The antibody has been used to establish an ELISA method for use in quantitating the F1 antigen. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgA |
| Name of Depositor |
MK Gentry, JE Williams |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Gentry MK, et al. Use of an enzyme-linked immunosorbent assay to measure antigenaemia during acute plague. Bull. World Health Organ. 62: 463-466, 1984. PubMed: 6380787
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