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F5-A-1/22.8.13
F5-A-1/22.8.13
規(guī)格:
貨期:
編號(hào):B164430
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 F5-A-1/22.8.13
商品貨號(hào) B164430
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified PA.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human prostate antigen (PA)
Cellular Products
immunoglobulin; monoclonal antibody; against human prostate antigen (PA)
Comments
Animals were immunized with purified PA.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Complete Growth Medium
Culture medium: RPMI 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype IgM; kappa light chain
Name of Depositor Research Corporation
U.S. Patent Number
References

Wang MC, et al. Purified human prostate antigen. US Patent 4,446,122 dated May 1 1984

Wang MC, et al. Purification of a human prostate specific antigen. Invest. Urol. 17: 159-163, 1979. PubMed: 89106

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