| 產(chǎn)品名稱 |
FHCR-1-2624/FH6/FHOT-1-3019 [FH6] |
| 商品貨號 |
B164455 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Adenocarcinoma,Cancer |
| Applications |
The antibody reacts with the 6B ganglioside and not with other structurally related gangliosides. The FH6 antibody reacts with a variety of human cancer cells, but does not react with normal human tissues. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified 6B ganglioside mixed with Salmonella minnesota. Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against 6B ganglioside, Lex, (tumor [adenocarcinoma] associated fucoganglioside) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against 6B ganglioside, Lex, (tumor [adenocarcinoma] associated fucoganglioside) |
| Comments |
Animals were immunized with purified 6B ganglioside mixed with Salmonella minnesota. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with the 6B ganglioside and not with other structurally related gangliosides. The 6B ganglioside is absent in normal colon tissue, but accumulates in adenocarcinomas of the colon. The FH6 antibody reacts with a variety of human cancer cells, but does not react with normal human tissues. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 88%; fetal bovine serum, 12%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
Fred Hutchinson Cancer Res. Cntr. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Hakomori S. Hybridoma antibody (FH6) defining a human cancer-associated difucoganglioside. US Patent 4,904,596 dated Feb 27 1990
Kim YS, et al. Lex and Ley antigen expression in human pancreatic cancer. Cancer Res. 48: 475-482, 1988. PubMed: 3335015
Nakasaki H, et al. Mosaicism in the expression of tumor-associated carbohydrate antigens in human colonic and gastric cancers. Cancer Res. 49: 3662-3669, 1989. PubMed: 2659167
Rosenman SJ, et al. Murine embryonal carcinoma cell-surface sialyl LeX is present on a novel glycoprotein and on high-molecular-weight lactosaminoglycan. Exp. Cell Res. 180: 326-340, 1989. PubMed: 2464500
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