| 產(chǎn)品名稱 |
FHM |
| 商品貨號(hào) |
B164457 |
| Organism |
Pimephales promelas, fathead minnow |
| Tissue |
connective tissue and muscle |
| Cell Type |
epithelial |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The FHM (Fat Head Minnow) line of epithelial cells was derived from tissue posterior to the anus, exclusive of the caudal fin, of normal adult minnows of both sexes by M. Gravell and R.G. Malsberger, in April, 1962. |
| Virus Susceptibility |
Infectious pancreatic necrosis virus
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
|
| Virus Resistance |
poliovirus 1 |
| Comments |
The cells multiply over a wide range of temperatures (0 to 36°C) with optimal growth between 28°C and 34°C. |
| Complete Growth Medium |
Minimum essential medium (Eagle) with Hank’s balanced salts (Life Technologies Cat# 11575-032), 90%; fetal bovine serum, 10% |
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and allow the flask to sit at room temperature. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 34°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: At the time of subcultivation (this will vary depending upon the temperature of incubation) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 100%
Temperature: 34°C; (Max. 34°C, Min. 28°C) |
| Name of Depositor |
RG Malsberger |
| Deposited As |
Pimephales promelas |
| Year of Origin |
April, 1962 |
| References |
Gravell M, Malsberger RG. A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 126: 555-565, 1965. PubMed: 5220177
Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hyg. 78: 173-180, 1963. PubMed: 14063721
|
