產(chǎn)品名稱 |
FHs 74 Int |
商品貨號(hào) |
B164460 |
Organism |
Homo sapiens, human |
Tissue |
small intestine |
Product Format |
frozen |
Morphology |
epithelial |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
normal |
Age |
3 to 4 months gestation |
Gender |
female |
Storage Conditions |
liquid nitrogen vapor phase |
Karyotype |
normal female; diploid |
Images |
|
Clinical Data |
female |
Receptor Expression |
epidermal growth factor (EGF), expressed |
Tumorigenic |
No |
Effects |
No, in immunosuppressed mice Yes, in semisolid medium |
Comments |
PAS, negative. The cells are negative for keratin by immunoperoxidase staining. |
Complete Growth Medium |
Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10% (Do not filter the medium after addition of EGF)
|
Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase |
Culture Conditions |
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
STR Profile |
Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 11,12 D5S818: 11,12 D7S820: 9,10 THO1: 7,9.3 TPOX: 8 vWA: 16,18 |
Isoenzymes |
AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 1 PGM3, 1 |
Name of Depositor |
R Owens |
Deposited As |
Homo sapiens |
References |
Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205
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