Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
Lymphoma
Strain
Strain: Wistar (B cell); BALB/c (myeloma)
Tissue: B lymphocyte; hybridoma
Morphology: lymphoblast
Depositors: E.C. Butcher; D. Andrew
Products: immunoglobulin; monoclonal antibody; against murine b7 integrin
FreezeMedium: Culture medium, 95%; DMSO, 5%
FluidRenewal: Every 2 to 3 days
SubCulturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/mL and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/mL.
Growth Properties: suspension
Comments: Animals were immunized with cells of the TK1 murine T cell lymphoma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with human and mouse b7 integrin. It partially blocks lymphocyte binding to MAdCAM-1, and a4b7 integrin binding to VCAM-1 and fibronectin. It is useful for both immunofluorescence and immunohistological staining. NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state.
References:
????????? RF33063: Berlin C et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993 PubMed: 93327426
????????? RF33868: Andrew DP et al. Distinct but overlapping epitopes are involved in a4b7 - mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994 PubMed: 95015875
Applications
The antibody reacts with human and mouse beta 7 integrin.
It is useful for both immunofluorescence and immunohistological staining.
NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine beta 7 integrin
Cellular Products
immunoglobulin; monoclonal antibody; against murine beta 7 integrin
Comments
Animals were immunized with cells of the TK1 murine T cell lymphoma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with human and mouse beta 7 integrin.
It partially blocks lymphocyte binding to MAdCAM-1, and alpha 4, beta 7 integrin binding to VCAM-1 and fibronectin.
It is useful for both immunofluorescence and immunohistological staining.
NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
0.05 mM 2-mercaptoethanol
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
EC Butcher, DP Andrew
Deposited As
rat (B cell); mouse (myeloma)
References
Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523
Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506
