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FIGR
FIGR
規(guī)格:
貨期:
編號:B164463
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 FIGR
商品貨號 B164463
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the glucorticoid receptor (GR) of mouse and rat, but does not react with human GR.
The antibody produced by FIGR appears to be equivalent to that produced by the BUGR2 hybridoma in that the two antibodies will cross inhibit, and both bind to the same 16000 dalton tryptic fragment of GR.
The antibody is useful for immunopurification and Western blotting applications.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
The antibody produced by FIGR appears to be equivalent to that produced by the BUGR2 hybridoma in that the two antibodies will cross inhibit, and both bind to the same 16000 dalton tryptic fragment of GR.
Genes Expressed
immunoglobulin; monoclonal antibody; against glucocorticoid receptor of mouse and rat
Cellular Products
immunoglobulin; monoclonal antibody; against glucocorticoid receptor of mouse and rat
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Spleen cells were fused with NS-1 myeloma cells.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; iron supplemented bovine calf serum, 10%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly.
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor JE Bodwell
Deposited As mouse (B cell); mouse (myeloma)
References

Bodwell JE, et al. Identification of phosphorylated sites in the mouse glucocorticoid receptor. J. Biol. Chem. 266: 7549-7555, 1991. PubMed: 2019585

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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