| 產(chǎn)品名稱 | G-401 [G401] |
|---|---|
| 商品貨號 | B164483 |
| Organism | Homo sapiens, human |
| Tissue | kidney |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | rhabdoid tumor (formerly classified as Wilms' tumor) |
| Age | 3 months |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor temperature |
| Karyotype | The cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation. |
| Images |  |
| Derivation | G-401 was deposited as a cell line derived from a Wilms' tumor. |
| Clinical Data | 3 months Caucasian male |
| Genes Expressed | nephroblast growth factor (NB-GF) |
| Cellular Products | nephroblast growth factor (NB-GF) |
| Tumorigenic | Yes |
| Effects | Yes, forms colonies in soft agar |
| Comments | G-401 was deposited as a cell line derived from a Wilms' tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. Ref   Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007 |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 9,14 D16S539: 12 D5S818: 13 D7S820: 11,14 THO1: 8,9.3 TPOX: 8,11 vWA: 16 |
| Isoenzymes | G6PD, B |
| Name of Depositor | PT Peebles |
| Deposited As | Homo sapiens |
| References | Weissman BE, et al. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression. Science 236: 175-176, 1987. PubMed: 3031816 Burrow CR, Wilson PD. A putative Wilms tumor-secreted growth factor activity required for primary culture of human nephroblasts. Proc. Natl. Acad. Sci. USA 90: 6066-6070, 1993. PubMed: 8392186 Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614 Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007 |
| Cross References | Nucleotide (GenBank) : D10232 Homo sapiens mRNA for renin-binding protein, complete cds. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
