產(chǎn)品名稱 |
G-401 [G401] |
商品貨號 |
B164483 |
Organism |
Homo sapiens, human |
Tissue |
kidney |
Product Format |
frozen |
Morphology |
epithelial |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
rhabdoid tumor (formerly classified as Wilms' tumor) |
Age |
3 months |
Gender |
male |
Ethnicity |
Caucasian |
Storage Conditions |
liquid nitrogen vapor temperature |
Karyotype |
The cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation. |
Images |
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Derivation |
G-401 was deposited as a cell line derived from a Wilms' tumor.
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Clinical Data |
3 months Caucasian male |
Genes Expressed |
nephroblast growth factor (NB-GF) |
Cellular Products |
nephroblast growth factor (NB-GF) |
Tumorigenic |
Yes |
Effects |
Yes, forms colonies in soft agar |
Comments |
G-401 was deposited as a cell line derived from a Wilms' tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. Ref Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007 |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
Cryopreservation |
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature |
Culture Conditions |
Temperature: 37°C |
STR Profile |
Amelogenin: X,Y CSF1PO: 11,13 D13S317: 9,14 D16S539: 12 D5S818: 13 D7S820: 11,14 THO1: 8,9.3 TPOX: 8,11 vWA: 16 |
Isoenzymes |
G6PD, B |
Name of Depositor |
PT Peebles |
Deposited As |
Homo sapiens |
References |
Weissman BE, et al. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression. Science 236: 175-176, 1987. PubMed: 3031816
Burrow CR, Wilson PD. A putative Wilms tumor-secreted growth factor activity required for primary culture of human nephroblasts. Proc. Natl. Acad. Sci. USA 90: 6066-6070, 1993. PubMed: 8392186
Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614
Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007
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Cross References |
Nucleotide (GenBank) :
D10232
Homo sapiens mRNA for renin-binding protein, complete cds.
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