產(chǎn)品名稱 |
GDM-1 |
商品貨號 |
B164499 |
Organism |
Homo sapiens, human |
Tissue |
peripheral blood |
Cell Type |
monoblast |
Product Format |
frozen |
Morphology |
lymphoblast |
Culture Properties |
suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
myelomonoblastic leukemia |
Age |
66 - 67 years |
Gender |
female |
Ethnicity |
White |
Applications |
This cell line is a model system for studying myelomonocytic disorders and leukemias. |
Storage Conditions |
liquid nitrogen vapor temperature |
Derivation |
The GDM-1 cell line was established in 1980 from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia. |
Clinical Data |
66 - 67 years
female
White |
Antigen Expression |
Ia +; myeloid leukemia antigen (M-1) Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |
Receptor Expression |
complement (C3) Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552Fc Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |
Genes Expressed |
lysozyme
Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552
myeloperoxidase
Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552
|
Cellular Products |
lysozyme
Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552
myeloperoxidase
Ref Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552 |
Tumorigenic |
No |
Effects |
No, did not form colonies in soft agar |
Comments |
The cells lack B- and T-cell surface markers including T-associated antigens, E-rosetting capacity, surface and intracytoplasmic immunoglobulins. They are non-specific esterase positive. The cells are phagocytic for latex beads and iron particles. Exposure to phorbol 12-myristate 13-acetate (TPA) increases the phagocytic activity. TPA also induces macrophage-like differentiation. The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 x 10 5 viable cells/mL. Maintain cultures at cell concentrations between 3 x 10 5 and 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
|
Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
Culture Conditions |
Temperature: 37°C
|
Population Doubling Time |
24 to 36 hrs |
Name of Depositor |
H Ben-Bassat |
Deposited As |
human |
Year of Origin |
1980 |
References |
Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552
|