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H18/7
H18/7
規(guī)格:
貨期:
編號(hào):B164524
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 H18/7
商品貨號(hào) B164524
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with E-selectin and blocks leukocyte binding to endothelial cells.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human E-selectin (ELAM-1, CD62E)
Cellular Products
immunoglobulin; monoclonal antibody; against human E-selectin (ELAM-1, CD62E)
Comments
Animals were immunized with human endothelial cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with E-selectin and blocks leukocyte binding to endothelial cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
RPMI 1640 medium, 70%, horse serum, 20%; DMSO, 10% 
Culture Conditions
Temperature: 37.0°C
Isotype Secretes a mouse IgG2a monoclonal antibody that reacts with human E-selectin
(ELAM-1, CD62E).
Name of Depositor M Gimbrone, Brigham and Women's Hospital
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Bevilacqua MP, et al. Identification of an inducible endothelial-leukocyte adhesion molecule. Proc. Natl. Acad. Sci. USA 84: 9238-9242, 1987. PubMed: 2827173

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