国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > H3.1
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
H3.1
H3.1
規(guī)格:
貨期:
編號(hào):B164532
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 H3.1
商品貨號(hào) B164532
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Strain BALB/c
Applications
ELISA detection zona pellucida glycoprotein 3 zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C
Western Blot zona pellucida glycoprotein 3 zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C
detection of zona pellucida glycoprotein 3 zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
zona pellucida glycoprotein 3
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium at 1 to 2 x 105 viable cells/mL. Add medium as the cell density increases. Maintain cultures at cell concentrations between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days. Scrape attached cells if necessary.

Cryopreservation Fetal bovine serum, 90%; DMSO, 10% (v/v). Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1 kappa
Name of Depositor J Dean
Deposited As mouse (B cell); mouse (myeloma)
References

Rankin TL, et al. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125: 2415-2424, 1998. PubMed: 9609824

Greenhouse S, et al. Antibodies to human ZP3 induce reversible contraception in transgenic mice with 'humanized' zonae pellucidae. Hum. Reprod. 14: 593-600, 1999. PubMed: 2118547

Animals were immunized with a human zona pellucida 3 (ZP3) peptide with a C-terminal cysteine (RQPHVMSQWSRSASRNC) coupled to keyhole limpet haemocyanin. The zona pellucida is an extracellular matrix that mediates critical steps in fertilization and early development surrounds all vertebrate eggs, and in mice and humans comprises three glycoproteins: ZP1, ZP2 and ZP3.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479