| 產(chǎn)品名稱 | HCC2157 |
|---|---|
| 商品貨號 | B164571 |
| Organism | Homo sapiens, human |
| Tissue | mammary gland; breast |
| Cell Type | Epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | suspension, multicell aggregates, Cells in suspension show an inclination to form duct-like structures and gland-like structures. |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | TNM stage IIIA, grade 2,primary ductal carcinoma |
| Age | 48 years adult |
| Gender | female |
| Ethnicity | Black |
| Applications | HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). The cells are positive for expression of Her2-neu and for expression of p53. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). |
| Storage Conditions | liquid nitrogen vapor temperature |
| Karyotype | Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% |
| Images |  |
| Derivation | This cell line was initiated on 3/4/96 and took 8 months to establish.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341. |
| Clinical Data | Black
female
48 years
|
| Receptor Expression | estrogen receptor, negative progesterone receptor, positive |
| Oncogene | her2/neu +, p53 + |
| Genes Expressed | Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 |
| Cellular Products | Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 |
| Comments | The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341. |
| Complete Growth Medium | RPMI-1640 medium, 95%; fetal bovine serum, 5% |
| Subculturing | Cultures can be maintained by addition of fresh medium. Cultures grow as floating aggregates of large irregular cell clusters. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 11 D5S818: 13 D7S820: 10,12 THO1: 8,9 TPOX: 9,11 vWA: 14,18 |
| Name of Depositor | AF Gazdar, AK Virmani |
| Year of Origin | 1996 |
| References | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
