| 產(chǎn)品名稱 | HCC38 |
|---|---|
| 商品貨號 | B164574 |
| Organism | Homo sapiens, human |
| Tissue | mammary gland; breast/duct |
| Cell Type | Epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent, single cells and loosely attached clusters |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | TNM stage IIB, grade 3, primary ductal carcinoma |
| Age | 50 years adult |
| Gender | female |
| Ethnicity | Caucasian, White |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% HCC38 contains a homozygous deletion at 3p12. At least 45 distinct derivative chromosomes were detected in most metaphases, including two large metacentric markers which are approximately 1.5 times longer than a normal A group chromosome. Other derivative chromosomes: del(1)(q24); del(1)(p22); del(1)(p13); add(1)(p12); del(2)(p16); add(3)(q10); del(3)(q13); ?del(4)(q13) (two copies per cell); del(5)?(q23q33); der (7)(pter?q11::?hsr); del(7)(p?); del(9)(p12); add(9)(p10); ?der(11); add(12)(q24)(very long); add(14)(p11); add(17)(p12); der(18); der(X); plus approx. 17-24 markers of unknown origin per cell. This is a hyper-triploid human cell line with a modal chromosome number of 75. Homogeneously staining regions and dicentric chromosomes were observed. Every chromosome pair had a least one rearrangement. No normal X chromosomes were observed and Y chromosomes were absent by QM staining. The following structural rearrangements were observed in 30 metaphases: an acentric fragment in 2/30 metaphases, a minute in 3/30, a chromosome break in 3/30, a chromatid break in 5/30, a ring chromosome in 1/30, and double minutes in 11/30 (1-5 copies). Pulverized chromosomes were reported in 5% of metaphases. C-banding revealed that several of the large markers are dicentric. No normal X chromosomes were observed and Y chromosomes were absent by QM staining. Normal copies of chromosomes 2,6,11,13,16 and 20 were seen. Composite karyotype: 65-79<3n> der(X), -1,-1,-1, del(1)(q24), del(1)(p22),del(1)(p13),add(1)(p12),-2,del(2)(p16)x2,-3,-3,-3, ?add(3)(q10),del(3)(q13)x2,-4,-4,-4, ?del(4)(q13)x2,-5, -5,-5,del(5)?(q23q33)x2,-6,-6,-7,-7,-7,der (7)(pter?q11::?hsr), del(7)(p?)x2,-8,-8,-8,-9,-9,-9,del(9)(p12)x2,add(9)(p10)x2, -10,-10,-10,-11,?der(11),-12,-12,-12, add(12)(q24), -13,-13,-14,-14,-14,add(14)(p11)x2,-15,-15,-15,-17,-17,-17, add(17)(p12)x2,-18,-18,-18,der(18),-19,-19,-19,-21,-21, -22,-22,+17 to 24 mar[cp11]. |
| Images |  |
| Derivation | HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. This cell line was initiated on 4/27/92 and took 32 months to establish. |
| Clinical Data | 50 years
Caucasian, White
female
|
| Receptor Expression | estrogen receptor, not expressed progesterone receptor, not expressed |
| Oncogene | her2/neu -; p53 + |
| Genes Expressed | Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 |
| Cellular Products | Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 |
| Comments | HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). The cells are negative for expression of Her2-neu but positive for expression of p53 The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.
|
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X CSF1PO: 12 D13S317: 12,14 D16S539: 10,14 D5S818: 9 D7S820: 10 THO1: 9.3 TPOX: 9,12 vWA: 16,17 |
| Name of Depositor | AF Gazdar, AK Virmani |
| Year of Origin | April 27, 1992 |
| References | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 Sundarsan V, et al. Homozygous deletions at 3p12 in breast and lung cancer. Oncogene 17: 1723-1729, 1998. PubMed: 9796701 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
