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HCC70
HCC70
規(guī)格:
貨期:
編號(hào):B164576
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) HCC70
商品貨號(hào) B164576
Organism Homo sapiens, human
Tissue
mammary gland; breast/duct
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent, The line grows as attached medium-sized epithelial cells without floating cells.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease TNM stage IIIA, grade 3,primary ductal carcinoma
Age 49 years
Gender female
Ethnicity Black
Applications
HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells.
The cells are poorly differentiated.
The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.
The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Storage Conditions liquid nitrogen vapor phase
Karyotype polyploid
Images ATCC CRL-2315 Micrograph
Derivation
The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.
Clinical Data
49 years
Black
female
Receptor Expression
progesterone receptor, not expressed
Oncogene her2/neu -, p53 + (overexpressed)
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19,her2/neu -, p53 + (overexpressed),The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.,The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments
The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.
The cells are poorly differentiated.
The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 17 lymph nodes.
The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.
HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. An independent study, by ATCC Scientists, is underway to assess the estrogen receptor status; results of this study will be published online upon completion.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin:X
CSF1PO:10,14
D13S317:12
D16S539:9,13
D5S818:12,13
D7S820:10,11
THO1:9
TPOX:10
vWA: 13,15
Name of Depositor AF Gazdar, AK Virmani
Deposited As Homo sapiens
Year of Origin 1992
References

Kao J, et al. Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery. PlosONE 4 (7): e 6146, 2009

Kim MS, et al. Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform. PlosONE 5 (5): e10441, 2010

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479