產(chǎn)品名稱 |
HCT-15 |
商品貨號 |
B164581 |
Organism |
Homo sapiens, human |
Tissue |
colon |
Product Format |
frozen |
Morphology |
epithelial |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
Dukes' type C, colorectal adenocarcinoma |
Gender |
male |
Applications |
This cell line is a suitable transfection host. |
Storage Conditions |
liquid nitrogen vapor phase |
Karyotype |
This is a quasidiploid human cell line with the modal number 46 occurring in 76% of cells (range = 41 to 47 for 50 metaphases).The rate of polyploidy was 5.1%. The karyotype of the line 46, XY, -8,-11, -17, t(8:17)(p23:q21), inv(11)(p15.3q13.1). The Y chromosome was slightly longer than N22, and had a large segment of heterochromatic, fluorescent distal q arms. |
Images |
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Derivation |
Evidence from DNA fingerprinting indicates that this line and DLD-1 (ATCC CCL-221) are derived from the same individual; however, isoenzymology and cytogenetic data leave some doubt. |
Clinical Data |
male |
Genes Expressed |
carcinoembryonic antigen (CEA) 5.4 ng/106 cells/10 days. The cells are positive for keratin by immunoperoxidase staining. |
Cellular Products |
carcinoembryonic antigen (CEA) 5.4 ng/106 cells/10 days and keratin |
Tumorigenic |
Yes |
Effects |
Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
Comments |
HCT-15 cells are CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining.
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Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
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Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
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Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
Culture Conditions |
Temperature: 37°C |
STR Profile |
Amelogenin: X,Y CSF1PO: 12 D13S317: 8,11 D16S539: 12,13 D5S818: 13 D7S820: 10,12 THO1: 7,9.3 TPOX: 8,11 vWA: 18,19 |
Isoenzymes |
ES-D, 2 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1 |
Name of Depositor |
DL Dexter |
Deposited As |
Homo sapiens |
References |
Chen TR, et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 7621404
Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
Dexter DL, et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 427742
Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064
Vermeulen SJ, et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040
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