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HEC-1-A
HEC-1-A
規(guī)格:
貨期:
編號:B164586
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HEC-1-A
商品貨號 B164586
Organism Homo sapiens, human
Tissue uterus; endometrium
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease adenocarcinoma
Age 71 years
Gender female
Karyotype hypodiploid to hyperdiploid, modal number = 47 with large metacentric marker
Derivation
This line and a substrain HEC-1-B (ATCC HTB-113) were isolated in 1968 by H. Kuramoto and associates from a patient with stage IA endometrial cancer.
Clinical Data
female
Antigen Expression
Blood Type B; Rh+
Receptor Expression
Receptor expression: platelet activating factor (PAF)
Oncogene c-fos +
Genes Expressed
Oncogenes: c-fos +
Tumorigenic Yes
Effects
Yes, in nude mice
Yes, in the cheek pouch of cortisone treated hamsters
Comments
PAF induces increased expression of c-fos.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
Name of Depositor H Kuramoto
Deposited As Homo sapiens
References

Kuramoto H. Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Presta M, et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185

Maggi M, et al. Platelet-activating factor mediates an autocrine proliferative loop in the endometrial adenocarcinoma cell line HEC-1A. Cancer Res. 54: 4777-4784, 1994. PubMed: 7520361

Kuramoto H, et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II).

The cells form typical papillary adenomas.

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