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HECA-452
HECA-452
規(guī)格:
貨期:
編號(hào):B164588
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HECA-452
商品貨號(hào) B164588
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: Wistar

Applications
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Cellular Products
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Comments
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
The antibody also recognizes sialyl Lewis x and sialyl Lewis a antigens and other carbohydrate ligands for human E-selectin.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and non essential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype rat IgM
Name of Depositor Stanford University
Deposited As rat (B cell); mouse (myeloma)
U.S. Patent Number
References

Butcher EC, Picker LJ. Neutrophil LECAM-1 as indicator of neutrophil activation. US Patent 5,316,913 dated May 31 1994

Duijvestijn AM, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol. 130: 147-155, 1988. PubMed: 3276207

Berg EL, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J. Exp. Med. 174: 1461-1466, 1991. PubMed: 1720810

The antibody reacts with a 200-kd cell-surface glycoprotein present on a subset of peripheral blood T cells, including both CD4+ and CD8+ T cells.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479