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HFN 7.1
HFN 7.1
規(guī)格:
貨期:
編號(hào):B164603
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) HFN 7.1
商品貨號(hào) B164603
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Mice were immunized with purified human fibronectin.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against human fibronectin,H-2d
Cellular Products
immunoglobulin; monoclonal antibody; against human fibronectin
Comments
The antibody does not cross-react with other fibronectins tested (26 species including 15 primates).
Mice were immunized with purified human fibronectin.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC HB-39.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor RC Schoen, KL Bentley, RJ Klebe
Deposited As mouse (B cell); mouse (myeloma)
References

Schoen RC, et al. Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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