| 產(chǎn)品名稱 |
hIL-15-M111 |
| 商品貨號 |
B164613 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications |
Animals were immunized with yeast-derived human interleukin-15 (IL-15). The antibody reacts with IL-15, an epithelium derived T cell growth factor. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with yeast-derived human interleukin-15 (IL-15). Spleen cells were fused with AGX653 mouse myeloma cells. The antibody reacts with IL-15, an epithelium derived T cell growth factor. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against interleukin 15 (IL-15) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against interleukin 15 (IL-15) |
| Comments |
Animals were immunized with yeast-derived human interleukin-15 (IL-15). Spleen cells were fused with AGX653 mouse myeloma cells. The antibody reacts with IL-15, an epithelium derived T cell growth factor. Monoclonal antibodies generated can be assayed for IL-15 antagonist activity using the CTLL-2 assay as essentially described by Gillis, et al. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
Immunex Corporation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Gillis S, Smith KA. Long term culture of tumour-specific cytotoxic T cells. Nature 268: 154-156, 1977. PubMed: 145543
Grabstein KH, et al. Antagonists of interleukin-15. US Patent 5,795,966 dated Aug 18 1998
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