產(chǎn)品名稱 |
Hs 738.St/Int |
商品貨號 |
B164728 |
Organism |
Homo sapiens, human |
Tissue |
mixed; stomach; intestine |
Product Format |
frozen |
Morphology |
fibroblast |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
normal |
Age |
18 weeks gestation fetus |
Gender |
male |
Ethnicity |
Caucasian |
Storage Conditions |
liquid nitrogen vapor termperature |
Karyotype |
modal number = 46; range = 44 to 92 |
Images |
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Derivation |
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
Clinical Data |
18 weeks gestation fetus Caucasian male |
Comments |
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 2 to 3 days |
Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor termperature |
Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
STR Profile |
Amelogenin: X,Y CSF1PO: 10,11 D13S317: 8,11 D16S539: 9,11 D5S818: 11,13 D7S820: 8,12 THO1: 7,9.3 TPOX: 8 vWA: 16,18 |
Passage History |
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |