| 產(chǎn)品名稱 | Hs 929.Sk |
|---|---|
| 商品貨號 | B164779 |
| Organism | Homo sapiens, human |
| Tissue | skin |
| Product Format | flask |
| Morphology | mixed |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Granuloma |
| Age | 5 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 46 |
| Images |  |
| Derivation | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Clinical Data | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). male Caucasian 5 years |
| Comments | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. Note: This item is distributed only within the 50 United States. It is not available for international distribution. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37.0°C |
| Passage History | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
