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IgG-7D4
IgG-7D4
規(guī)格:
貨期:
編號:B164845
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 IgG-7D4
商品貨號 B164845
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against hamster sterol regulatory element binding protein 2 (SREBP 2, SREBP-2)
Cellular Products
immunoglobulin; monoclonal antibody; against hamster sterol regulatory element binding protein 2 (SREBP 2, SREBP-2)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes both the precursor and mature forms of hamster SREBP-2.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor JL Goldstein, YK Ho
Deposited As mouse (B cell); mouse (myeloma)
References

Yang J, et al. Three different rearrangements in a single intron truncate sterol regulatory element binding protein-2 and produce sterol-resistant phenotype in three cell lines. Role of introns in protein evolution. J. Biol. Chem. 270: 12152-12161, 1995. PubMed: 7744865

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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