產(chǎn)品名稱 |
Ja Bos |
商品貨號(hào) |
B164881 |
Organism |
Homo sapiens, human |
Tissue |
skin |
Product Format |
frozen |
Morphology |
fibroblast |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
Ehlers-Danlos syndrome, type II (hemorrhagic type) |
Age |
27 years |
Gender |
male |
Clinical Data |
The tissue donor suffered from type II Ehlers-Danlos syndrome (hemorrhagic type), and was the brother of Fe Bos (ATCC CRL-1177). male |
Comments |
The tissue donor suffered from type II Ehlers-Danlos syndrome (hemorrhagic type), and was the brother of Fe Bos (ATCC CRL-1177). A brother, sister and mother were affected with the same condition. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |