產(chǎn)品名稱 |
Ja Coo |
商品貨號 |
B164882 |
Organism |
Homo sapiens, human |
Tissue |
skin |
Product Format |
frozen |
Morphology |
fibroblast |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
osteogenesis imperfecta (tarda) |
Age |
27 years |
Gender |
female |
Clinical Data |
The tissue donor suffered from osteogenesis imperfecta (tarda), and was the mother of Am Coo (ATCC CRL-1286) and Pa Coo (ATCC CRL-1293). female |
Comments |
The tissue donor suffered from osteogenesis imperfecta (tarda), and was the mother of Am Coo (ATCC CRL-1286) and Pa Coo (ATCC CRL-1293). |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |