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JM1
JM1
規(guī)格:
貨期:
編號(hào):B164894
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 JM1
商品貨號(hào) B164894
Organism Homo sapiens, human
Tissue blood
Cell Type pre-B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease B cell lymphoma
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.
Antigen Expression
HLA-DR; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD10; Homo sapiens, expressed
Genes Expressed
CD19; Homo sapiens, expressed ,CD10; Homo sapiens, expressed ,HLA-DR; Homo sapiens, expressed
Comments
The cells are negative for CD7, My 7, My 9 and surface IgM.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 105 viable cells/mL. Subculture when cell density reaches 1 x 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation Complete culture medium, 95%; DMSO 5%. Cell culture tested DMSO is available as ATCC? Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
D5S818: 12
D13S317: 11
D7S820: 8, 10
D16S539: 9
vWA: 17
THO1: 6
TPOX: 10, 11
CSF1PO: 12
Amelogenin: X Y
Name of Depositor Immunex Corporation
Deposited As Homo sapiens
U.S. Patent Number
References

Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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