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KM81
KM81
規(guī)格:
貨期:
編號(hào):B164922
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) KM81
商品貨號(hào) B164922
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
KM81 recognizes an epitope close to, but distinct from, those detected by KM201 and KM114 (see ATCC TIB-240 and ATCC TIB-241).
Derivation
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Cellular Products
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Comments
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody blocks adhesion of a B cell hybridoma (BM-2) to BMS2 cells or to hyaluronate coated dishes.
The antibody prevents growth of lymphoid and myeloid cells in long term bone marrow cultures.
KM81 recognizes an epitope close to, but distinct from, those detected by KM201 and KM114 (see ATCC TIB-240 and ATCC TIB-241).
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor PW Kincade
Deposited As rat (B cell); mouse (myeloma)
References

Miyake K, et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J. Exp. Med. 171: 477-488, 1990. PubMed: 2406365

Miyake K, et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med. 172: 69-75, 1990. PubMed: 2193100

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