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L5.1
L5.1
規(guī)格:
貨期:
編號(hào):B164945
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 L5.1
商品貨號(hào) B164945
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease leukemia
Strain Strain: BALB/c (B cell); BALB/c (myeloma)
Applications
The antibody reacts strongly with all morphologically recognizable precursors of the human erythroid lineage.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human transferrin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human transferrin receptor
Comments
Animals were immunized with the HL-60 promyelocytic leukemia cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody precipitates an 87000 dalton glycoprotein present on the surface of K562 leukemia cells.
The antibody reacts strongly with all morphologically recognizable precursors of the human erythroid lineage.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor G Rovera
Deposited As mouse (B cell); mouse (myeloma)
References

Lebman D, et al. A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells. Blood 59: 671-678, 1982. PubMed: 7059675

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