| 產(chǎn)品名稱 |
LS 180 |
| 商品貨號 |
B165008 |
| Organism |
Homo sapiens, human |
| Tissue |
colon |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Dukes' type B, colorectal adenocarcinoma |
| Age |
58 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype |
modal number = 45; range = 42 to 47.
The stemline karyotype was 45,t(X;5) (q12;q31) with tetraploid-like cells occurring at 2.2 percent. Occasional changes in karyotypes were also noted as a result of balanced translocations producing new marker chromosomes. In conjunction with this, decreases in chromosomes involving interchromosomal exchanges were seen. The karyotype was exactly the same as that originally described by the depositor. |
| Clinical Data |
female
58 years
Caucasian |
| Antigen Expression |
serologically defined colon cancer antigen 3; Homo sapiens, expressed HLA A2, B13, B50; Blood type O |
| Genes Expressed |
carcinoembryonic antigen (CEA), interleukin 10, interleukin 6, mucin
The production of CEA in the ATCC seed stock was 1916 ng per 10(6) cells in 10 days.
|
| Cellular Products |
carcinoembryonic antigen interleukin 10 interleukin 6 mucin |
| Tumorigenic |
Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells |
| Effects |
Yes, in nude mice |
| Comments |
This line has never been trypsinized; however, the variant LS 174T (ATCC CL-188) has been subcultured by trypsinization. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove most medium. Dislodge cells from the floor of the flask by banging flask, washing cells by repeat pipetting and scraping gently. Aspirate and dispense into new flasks. These cells have never been trypsinized.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Two to three times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
|
| STR Profile |
Amelogenin: X CSF1PO: 10,13 D13S317: 10 D16S539: 11,13 D5S818: 10,15 D7S820: 11,9.3 THO1: 6,7 TPOX: 8,9 vWA: 15,18 |
| Isoenzymes |
ADA, 1 ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 |
| Name of Depositor |
Northwestern University |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| References |
Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041
Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980
Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615
Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757
Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228
Schuetz EG, et al. P-glycoprotein: a major determinant of rifampicin-inducible expression of cytochrome P4503A in mice and humans. Proc. Natl. Acad. Sci. USA 93: 4001-4005, 1996. PubMed: 8633005
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