| 產(chǎn)品名稱 |
MD |
| 商品貨號(hào) |
B165116 |
| Organism |
Homo sapiens, human |
| Tissue |
spleen |
| Cell Type |
monocyte/macrophage |
| Product Format |
frozen |
| Morphology |
monocyte/macrophage |
| Culture Properties |
loosely adherent, multicell aggregates |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The MD cell line was established from cryopreserved human spleen cells. |
| Comments |
Epstein-Barr virus has been detected in this cell line. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 105 viable cells/mL. A maximum of 1 to 2 X 106 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 11,15 D5S818: 11,13 D7S820: 10,11 THO1: 8 TPOX: 8,11 vWA: 16,17 |
| Name of Depositor |
E.I. du Pont de Nemours & Co. |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| References |
Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995
|
