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MPC 11 OUAr
MPC 11 OUAr
規(guī)格:
貨期:
編號(hào):B165188
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) MPC 11 OUAr
商品貨號(hào) B165188
Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease plasmacytoma; myeloma
Strain BALB/c
Applications
This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse).
Tested and found negative for ectromelia virus (mousepox).
Derivation
This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse).
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody,H-2d
Cellular Products
immunoglobulin; monoclonal antibody
Comments
This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor M Cohn
Deposited As Mus musculus
References

Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836

Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146

Gefter ML, et al. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet. 3: 231-236, 1977. PubMed: 605383

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

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