| 產(chǎn)品名稱(chēng) |
MRSS-1 (D2D4) |
| 商品貨號(hào) |
B165195 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells. The antibody cross-reacts with double stranded DNA and polyinosinic-cytidylic acid (poly I-C). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells. |
| Clinical Data |
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against DNA (single stranded) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against DNA (single stranded) |
| Comments |
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells. The antibody cross-reacts with double stranded DNA and polyinosinic-cytidylic acid (poly I-C). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG3 |
| Name of Depositor |
BH Hahn, F Ebling |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hahn BH, et al. Production of monoclonal murine antibodies to DNA by somatic cell hybrids. Arthritis Rheum. 23: 942-945, 1980. PubMed: 6157395
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