| 產(chǎn)品名稱 |
mSXL 104 |
| 商品貨號(hào) |
B165199 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with epitopes expressed on the female specific sex lethal gene product. The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras. It does not perform well in immunoprecipitation applications, but is very good when used as a probe for immunoblots. |
| Derivation |
Animals were immunized with purified LacZ - Sxl fusion protein. Spleen cells were fused with Sp2/0 myeloma cells. |
| Clinical Data |
The antibody reacts with epitopes expressed on the female specific sex lethal gene product. The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl) |
| Comments |
Animals were immunized with purified LacZ - Sxl fusion protein. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with epitopes expressed on the female specific sex lethal gene product. The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras. The antibody does not cross-react with extracts of HeLa cells or the common house fly (Musca domestica). It does not perform well in immunoprecipitation applications, but is very good when used as a probe for immunoblots. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: 1 to 2 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
D Bopp, P Schedl |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Bopp D, et al. Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster. Genes Dev. 5: 403-415, 1991. PubMed: 1900493
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