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mSXL 5
mSXL 5
規(guī)格:
貨期:
編號:B165202
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 mSXL 5
商品貨號 B165202
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with epitopes expressed on the female specific sex lethal gene product.
The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras.
The antibody cross-reacts strongly with antigens present in extracts of HeLa cells (31000 dalton and 45000 dalton) and with a 41000 dalton antigen in extracts of the common house fly (Musca domestica).
Derivation
Animals were immunized with purified LacZ - Sxl fusion protein.
Spleen cells were fused with Sp2/0 myeloma cells.
Clinical Data
The antibody reacts with epitopes expressed on the female specific sex lethal gene product.
The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras.
Genes Expressed
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl)
Cellular Products
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl)
Comments
Animals were immunized with purified LacZ - Sxl fusion protein.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with epitopes expressed on the female specific sex lethal gene product.
The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras.
The antibody cross-reacts strongly with antigens present in extracts of HeLa cells (31000 dalton and 45000 dalton) and with a 41000 dalton antigen in extracts of the common house fly (Musca domestica).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 1 to 2 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor D Bopp, P Schedl
Deposited As mouse (B cell); mouse (myeloma)
References

Bopp D, et al. Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster. Genes Dev. 5: 403-415, 1991. PubMed: 1900493

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