| 產(chǎn)品名稱(chēng) |
Mv 1 Lu (NBL-7) |
| 商品貨號(hào) |
B165205 |
| Organism |
Neovison vison, American Mink |
| Tissue |
lung |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
near term fetus |
| Gender |
male and female mixed |
| Applications |
This cell line is a suitable transfection host and is useful for focus forming assays for murine and feline sarcoma viruses. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population., Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population. |
| Derivation |
The Mv 1 Lu (NBL-7) cell line was initiated by A.J. Kniazeff, W.A. Nelson-Rees and N.B. Darby, Jr., in May, 1964, from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink. |
| Clinical Data |
male and female mixed |
| Virus Susceptibility |
Herpes simplex virus
Reovirus 3
Vaccinia virus
Vesicular stomatitis New Jersey virus
|
| Virus Resistance |
adenovirus 5; coxsackievirus A9, B5; poliovirus 2 |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
AJ Kniazeff |
| Year of Origin |
May, 1964 |
| References |
Henderson IC, et al. Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. Virology 60: 282-287, 1974. PubMed: 4366800
Miller AD, Chen F. Retrovirus packaging cells based on 10AI murine leukemia virus for production of vectors that use multiple receptors for cell entry. J. Virol. 70: 5564-5571, 1996. PubMed: 8764070
Siess DC, et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675
Wang H, et al. Modulation of ecotropic murine retrovirus by N-linked glycosylation of the cell surface receptor/amino acid transporter. J. Virol. 70: 6884-6891, 1996. PubMed: 8794331
Feng XH, Derynck R. Ligand-independent activation of transforming growth factor (TGF) beta-signaling pathways by heteromeric cytoplasmic domains of TGF-beta receptors. J. Biol. Chem. 271: 13123-13129, 1996. PubMed: 8662796
|
| Cross References |
Nucleotide (GenBank) :
M64428
Mustela vison TI1 mRNA, complete cds.
Nucleotide (GenBank) :
J02248
mink cell focus-forming 247 provirus clone 1 ltr.
Nucleotide (GenBank) :
J02250
mink cell focus-forming 247 provirus clone 2 ltr(seg 1).
Nucleotide (GenBank) :
J02251
mink cell focus-forming 247 provirus clone 2 ltr(seg 2).
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