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NC-37
NC-37
規(guī)格:
貨期:
編號(hào):B165227
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 NC-37
商品貨號(hào) B165227
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Burkitt's lymphoma
Gender male
Applications

The consistency of the line's characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies such as being used as a target cell for EBV superinfection studies. It also has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 48; range = 40 to 51
Derivation Although deposited with the ATCC as a lymphoblastoid cell line established from a 34-year-old male caucasian, DNA fingerprinting has shown this line to be a derivative of Raji (see ATCC CCL-86), a Burkitt's lymphoma cell line.
Comments

Each cell contains an average of 60 EBV genome copies.

The cells are EBNA positive (EBNA+) and surface immunoglobulin negative (sIg-).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures at 5 x 105 viable cells/mL. A maximum density of up to 2 x 106 is possible.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 3 to 4 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13,14
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16
Isoenzymes
G6PD, B
Name of Depositor J Wolff
Deposited As Homo sapiens
Year of Origin February, 1967
References

Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017

Mayyasi SA, et al. The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499

The NC-37 cell line was reportedly initiated by W. Korol from peripheral blood from a donor whose serum was positive for EBV antibodies as determined by immunofluorescence and virus coating test.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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