產(chǎn)品名稱 |
NCI-H1417 [H1417] |
商品貨號(hào) |
B165263 |
Organism |
Homo sapiens, human |
Tissue |
lung |
Product Format |
frozen |
Culture Properties |
suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
stage E,carcinoma; classic small cell lung cancer |
Age |
61 years |
Gender |
female |
Ethnicity |
Caucasian |
Applications |
The line was established in May 1986. |
Images |
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Derivation |
The line was established in May 1986. |
Clinical Data |
The patient was a smoker. female Caucasian 61 years |
Comments |
The line was established in May 1986. The patient was a smoker. 50 pack years. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing |
Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by standing culture flask vertically until cell clusters settle to bottom of flask. Remove approximately 1/3 of the culture medium. Break up clusters by gently pipetting and add fresh medium. Expand cultures into new culture vessels as necessary.
Add fresh medium every 2 to 4 days (depending on cell density). |
Cryopreservation |
Culture medium, 95%; DMSO, 5% |
STR Profile |
Amelogenin: X CSF1PO: 11 D13S317: 8,11 D16S539: 12,13 D5S818: 12 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,11 vWA: 19 |
Name of Depositor |
AF Gazdar, JD Minna |
Deposited As |
Homo sapiens |
Year of Origin |
1986 |
References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
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