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Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cell clusters which should not be broken up (the cells lose viability when aggregates are broken up).
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

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Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.