產(chǎn)品名稱 |
NCI-H719 [H719] |
商品貨號 |
B165361 |
Organism |
Homo sapiens, human |
Tissue |
lung |
Product Format |
frozen |
Culture Properties |
suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
stage E,carcinoma; classic small cell lung cancer |
Age |
55 years |
Gender |
female |
Ethnicity |
Caucasian |
Applications |
The line was established in April 1984. |
Images |
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Derivation |
The line was established in April 1984. |
Clinical Data |
The patient was a smoker. female Caucasian 55 years |
Comments |
The line was established in April 1984. The patient was a smoker. 100 pack years. |
Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
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Cryopreservation |
Culture medium, 95%; DMSO, 5% |
Culture Conditions |
Temperature: 37.0°C |
STR Profile |
Amelogenin: X CSF1PO: 10,11 D13S317: 12 D16S539: 9 D5S818: 13 D7S820: 10 THO1: 8,9 TPOX: 8,11 vWA: 16,18 |
Name of Depositor |
AF Gazdar, JD Minna |
Deposited As |
Homo sapiens |
Year of Origin |
1984 |
References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
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