| 產(chǎn)品名稱 |
NCTC clone 2472 |
| 商品貨號 |
B165384 |
| Organism |
Mus musculus, mouse |
| Tissue |
subcutaneous connective tissue; areolar and adipose |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
sarcoma |
| Age |
82 days |
| Gender |
male |
| Strain |
C3H/HeN |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 52; range = 47 to 173, An extra long telocentric demonstrable in 100/100 cells. No metacentrics were observed. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation |
NCTC clone 2472 was derived in May, 1956, by K.K. Sanford, et al. from NCTC strain 1742 (Subline VII) of the parent NCTC clone 1328.
The parent line was derived by G.D. Likely et al. in November, 1950, from a culture of subcutaneous areolar and adipose connective tissue taken from a normal 82-day-old male C3H/HeN mouse. |
| Clinical Data |
male |
| Tumorigenic |
yes, In non-treated C3H/HeN mice, sarcomas developed within 12 days in 20/20 mice injected intramuscularly with 3.2 X 10(6) cells. In irradiated mice (425 r whole body), tumors developed within 12 days in 18/18 mice injected with 3.2 X 10(6) cells. |
| Effects |
Yes, In irradiated mice (425 r whole body) (Tumors developed within 12 days in 18/18 mice injected with 3.2 X 10(6) cells.) Yes, in untreated C3H/HeN mice (In non-treated C3H/HeN mice, sarcomas developed within 12 days in 20/20 mice injected intramuscularly with 3.2 X 10(6) cells.) |
| Virus Susceptibility |
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
|
| Virus Resistance |
poliovirus 3 |
| Comments |
While cultures of NCTC strain 1742 produced sarcomas in 97% of the mice injected, cultures of NCTC clone 2472 under similar conditions produced sarcomas in 65% of the mice injected.
NCTC clone 2472 has been compared with related clones, such as NCTC clone 2555 (ATCC CCL-12), and certain other cell lines with respect to neoplastic and physiologic properties, nutitional needs, karyology, enzyme activities, and rates of glycolysis.
NCTC clone 2472 cells have been cultured since origin in an antibiotic-free mixture of medium NCTC 109 (135) with 10% horse serum.
Plating efficiency is approximately 10% in the recommended culture medium.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
NCTC 135 medium, 90%; horse serum, 10%
|
| Subculturing |
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 3 times per week
|
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
KK Sanford |
| Deposited As |
Mus musculus |
| Year of Origin |
1956 |
| References |
Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477
Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507
Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880
Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337
Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776
Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340
Dupree, L. T. , et al. Influence of serum protein on determination of nutritional requirements of cells in culture, Exp. Cell Res., 28, 381 (1962)
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