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OML, clone 13C
OML, clone 13C
規(guī)格:
貨期:
編號(hào):B165441
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 OML, clone 13C
商品貨號(hào) B165441
Organism Aotus trivirgatus, monkey, Owl
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, the cells grow in dense clumps
Biosafety Level 2 Cells contain Herpesvirus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age adult
Gender female
Applications
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Storage Conditions liquid nitrogen vapor phase
Karyotype The cells exhibit a karyotype consistent with the species Aotus trivirgatus; the chromosome count was 54 with an XX sex chromosome constitution., Analysis revealed a G-banding pattern of the type designated karyotype I.
Derivation
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Clinical Data
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
female
Antigen Expression
CD20 +
Genes Expressed
CD20 +
Comments
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The resulting cells were cloned by limiting dilution.
CITES Permit
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition or replacement of medium. When replacing media, centrifuge cells and resuspend cell pellet in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time 24 hrs
Name of Depositor JG Scammell
Year of Origin 1995
References

Scammell JG, et al. An EBV-transformed owl monkey B-lymphocyte cell line. In Vitro Cell. Dev. Biol. Anim. 33: 88-91, 1997. PubMed: 9081215

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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