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PI 153/3
PI 153/3
規(guī)格:
貨期:
編號(hào):B165508
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 PI 153/3
商品貨號(hào) B165508
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Neuroblastoma,Leukemia
Applications
The antigen recognized by this antibody is present on fetal brain, B cell and Null cell leukemias but not on adult brain.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a glycoprotein expressed on human neuroectoderm derived tumors (neuroblastoma, retinoblastoma and glioblastoma)
Cellular Products
immunoglobulin; monoclonal antibody; against a glycoprotein expressed on human neuroectoderm derived tumors (neuroblastoma, retinoblastoma and glioblastoma)
Comments
Animals were immunized with the human neuroblastoma line IMR6.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antigen recognized by this antibody is present on fetal brain, B cell and Null cell leukemias but not on adult brain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgM
Name of Depositor R Kennett
Deposited As mouse (B cell); mouse (myeloma)
References

Kennett RH, Gilbert F. Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science 203: 1120-1121, 1979. PubMed: 424740

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