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Psi2 DAP
Psi2 DAP
規(guī)格:
貨期:
編號(hào):B165531
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Psi2 DAP
商品貨號(hào) B165531
Organism Mus musculus, mouse
Tissue embryo
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age embryo
Strain NIH/Swiss
Applications
This line produces a vector that can infect mouse cells.
Psi2 DAP cells produce a vector that encodes the human placental alkaline phosphatase gene and the neomycin resistance gene.
The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP).
Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo.
The cells may produce helper virus.
Lineage analysis and immortalization of neural cells via retrovirus vectors in Neuromethods, Molecular Neurobiological Techniques 16:117-219, Boulton, A.A., Baker, G.B. and Campagnoni, A.T., Eds.
Derivation
The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP).
Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo.
Genes Expressed
a human placental alkaline phosphatase transducing vector
Cellular Products
a human placental alkaline phosphatase transducing vector
Comments
This line produces a vector that can infect mouse cells.
Psi2 DAP cells produce a vector that encodes the human placental alkaline phosphatase gene and the neomycin resistance gene.
The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP).
Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo.
The cells may produce helper virus.
To assay for helper virus, and for other retrovirus techniques, see Cepko, C.L.
Lineage analysis and immortalization of neural cells via retrovirus vectors in Neuromethods, Molecular Neurobiological Techniques 16:117-219, Boulton, A.A., Baker, G.B. and Campagnoni, A.T., Eds., Humana Press, Clifton, NJ, 1989.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor CL Cepko
Deposited As Mus musculus
References

Fields-Berry SC, et al. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA 89: 693-697, 1992. PubMed: 1731342

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