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PU5-1.8 (PU5-1R)
PU5-1.8 (PU5-1R)
規(guī)格:
貨期:
編號(hào):B165538
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 PU5-1.8 (PU5-1R)
商品貨號(hào) B165538
Organism Mus musculus, mouse
Product Format frozen
Culture Properties suspension (some adherent cells)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoid tumor
Strain BALB/c
Applications
The line is sensitive to growth inhibition by LPS and PPD.
Tested and found negative for ectromelia virus (mousepox).
Receptor Expression
complement (C3)
Genes Expressed
lysozyme; granulocyte colony stimulating activity (CSA) inducible by LPS
Cellular Products
lysozyme; granulocyte colony stimulating activity (CSA) inducible by LPS
Comments
PU5-1.8 cells phagocytose latex beads and zymosan.
The cells are capable of antibody dependent lysis of both sheep erythrocytes and tumor cells.
The line is sensitive to growth inhibition by LPS and PPD.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Adherent cells can be recovered by scraping.
Name of Depositor P Ralph
Deposited As Mus musculus
References

Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429

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胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
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